Antispasmodic Activity of Petroleum Ether Extract of Seeds of Apium graveolens (PEESAG) on Histamine Induced Contraction in Guinea Pig’s Ileum

Background: Apium graveolens has been used in Unani system of medicine as anti inflammatory, uricosuric, diuretic, carminative, antidyslipidemic, abdominal discomfort etc.The aim of the present study was to determine the antispasmodic activity of A. graveolens in Guinea Pigs against histamine induced contractions. Materials and Methods: Antispasmodic activity of PEESAG at the doses of 100, 200, 300, 400, 500 mcg/ml, 1 mg/ml, 2 mg/ml and 3 mg/ml against histamine at the dose of 10 mcg/ml in guinea pigs. Result: The test drug produced antispasmodic effects 40%, 60%, 60%, 70%, 70% 80%, 90% and 100% at the different doses of 100, 200, 300, 400, 500 mcg/ml, 1 mg/ml, 2 mg/ml and 3 mg/ml respectively. Conclusion: This result suggests that the PEESAG revealed antispasmodic activity as dose dependent i.e. maximum antispasmodic effect on 3mg/ml of the dose.